HIV 1 & 2

Available in Card Format

The Acculine HIV 1 & 2 is a rapid immunochromatographic screening test for the detection of antibodies to HIV 1 & 2 in human serum, plasma or whole blood. The test device contains test strip immobilized with recombinant antigens of HIV1 at test line (1), HIV 2 recombinant antigen at test line (2) and control line reagent at (C) on nitrocellulose membrane and dried gold conjugate sensitized with HIV antigens and anti control line.

The specimen is applied to the sample well followed by addition of running buffer. The buffer facilitates the lateral flow of the sample and gold conjugate as well as promoting the immunologic binding of antibodies and antigen, if present in patient’s specimen. The HIV antibodies present in the patient’s specimen binds to the gold conjugate and make a immune complex. The complex migrates on the nitrocellulose membrane and is captured by the antigens immobilized in the Test line zone (1 & 2) and produces pink/purple band with varying intensity, proportional to the HIV 1& 2 antibodies in the sample. In the absence of HIV 1/2 antibodies, there is no pink/purple band in the TEST line zone (1&2). The liquid continues to migrate along the membrane and produces another pink/purple band in the CONTROL line zone (C), demonstrating that the reagents are functioning properly and validates the result.

Immobilization of proteins on nitro cellulose membrane

  • GP-41, GP 120 & p24 on test line 1 for capturing of HIV-1 antibodies.
  • GP-36 on test line 2 for capturing of HIV-2 antibodies.

 

 

 

Sensitivity: 99.5%

Specificity: 99.7%

Test Procedure

  • Allow the kit components & test specimen to come at room temperature if stored in refrigerator.
  • Remove as many as test devices required for the testing from pouch by tearing along notch provided.
  • Lay down the test device on flat surface and mention patient’s ID number.
  • Dispense one drop (20µl) of serum, plasma or whole blood using provided dropper or micro pipette into sample well (S).
  • Add 2-3 drops of running buffer using provided dropper bottle or dropper into sample well (S).
  • Read the result in result window within 5-20 minutes of specimen and running buffer addition.

For OEM please contact us at acculinediagnostics@gmail.com.